MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1344 . LYU03 Medium

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1344 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Taps 17.4 g 17.4 -
Yeast extract 0.1 g 0.1 -
Sodium acetate 1.0 g 1 12.191
Disodium succinate 1.0 g 1 6.171
Na2S2O3 x 5 H2O 0.1 g 0.1 0.403
CaCl2 x 2 H2O 75.0 mg 0.075 0.51
MgSO4 x 7 H2O 0.2 g 0.2 0.811
NH4Cl 0.5 g 0.5 9.348
KH2PO4 0.5 g 0.5 3.674
Na2-EDTA 5.0 mg 0.005 0.015
Trace elements (A) 1.0 ml - -
Neutralized sulfide solution (B) 30.0 ml - -
Bicarbonate solution (C) 30.0 ml - -
Vitamin solution (D) 1.0 ml - -
Vitamin B12
(0.01%) 		0.4 ml 4.0e-5 3.0e-5
Distilled water 1000.0 ml - -
1 pH 8.3
2 Mix ingredients vitamin B12, vitamin mixture, bicarbonate solution and neutralized sulfide solution. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers and autoclave at 121°C for 15 min. Separately autoclave neutralized sulfide solution under a N2 atmosphere. Sterilize Vitamin B12 solution, vitamin mixture, bicarbonate solution by filtration. Prior to inoculation, aseptically and anaerobically add vitamin B12 solution, vitamin mixture, bicarbonate solution and neutralized sulfide solution.

Trace elements (A) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.2 g 5.2 17.794
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 4 H2O 0.1 g 0.1 0.505
FeCl2 x 4 H2O 1.5 g 1.5 7.545
H3BO3 6.0 mg 0.006 0.097
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
Na2MoO4 x 2 H2O 188.0 mg 0.188 0.777
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
ZnCl2 70.0 mg 0.07 0.514
VOSO4 x 2 H2O 30.0 mg 0.03 0.151
Na2WO4 x 2 H2O 2.0 mg 0.002 0.006
NaHSeO3 2.0 mg 0.002 -
Deionized water 1000.0 ml - -
1 EDTA should be fully dissolved before adding the other components. Store at 4°C.

Neutralized sulfide solution (B) #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 1.5 g 15 62.453
Distilled water 100.0 ml - -
1 The sulfide solution is prepared in a vessel sealed with a butyl rubber.
2 Autoclave at 121°C for 15 min under a N2 atmosphere. After cooling to room temperature the pH is adjusted to about 7.3 by adding of sterile 2N H2SO4 drop-wise with a syringe without opening the bottle. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.

Bicarbonate solution (C) #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.5 g 83.333 991.981
Distilled water 30.0 ml - -

Vitamin solution (D) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5.00 mg 0.05 0.037
Biotin 5.00 mg 0.05 0.205
Pyridoxamine hydrochloride 0.01 g 0.1 0.489
p-Aminobenzoic acid 0.03 g 0.3 2.188
Niacin 0.05 g 0.5 4.061
Thiamine HCl 0.05 g 0.5 1.482
Distilled water 100.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 8.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Taps 17.4 -
NaHCO3 2.5 29.759
Sodium acetate 1 12.191
Disodium succinate 1 6.171
NH4Cl 0.5 9.348
Show more
CSV
JSON