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Main sol. 1355

Internal Solution-ID: S2728

Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.50 g 2.5 14.353
NaH2PO4 2.25 g 2.25 18.754
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
Visniac Trace Elements (1000x) , pH 6 1.00 ml - -
Distilled water 1000.00 ml - -
Agar , for solid medium 12.00 g 12 35.679
Methylamine
(0.2% v/v)
2.00 ml - -
1 Adjust to pH 6 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. After autoclaving add sterile methylamine (0.2% v/v) to the sterile main solution.
1355 HYPHOMICROBIUM MEDIUM