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Main sol. 1367

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Internal Solution-ID: S2755

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.10 g 0.1 0.735
(NH4)2SO4 0.10 g 0.1 0.757
MgSO4 x 7 H2O 0.10 g 0.1 0.406
CaCl2 x 2 H2O 0.02 g 0.02 0.136
Hutner's salts 1.00 ml - -
Vitamin solution 1.00 ml - -
Yeast extract 0.10 g 0.1 -
Glucose 0.10 g 0.1 0.555
Distilled water 1000.00 ml - -
1 Prepare the liquid medium without Staley´s vitamin solution and glucose. Adjust to pH 4.8 - 5.5. Dispense the medium into serum bottles. The gas phase is air:CO2 (95:5) and the liquid:gas phase should be 1:9. Sterilize by autoclaving. After autoclaving add the Staley´s vitamin solution (filter sterilised) and glucose (autoclaved) from sterile stock solutions.
1367 TELMATOCOLA MEDIUM