Main sol. 1369
Internal Solution-ID: S2761
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NaCl | 30.0 | g | 30 | 513.347 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| Na-acetate | 1.0 | g | 1 | 12.19 | |
| Na2S2O3 | 0.5 | g | 0.5 | 1.167 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| NaHCO3 | 5.0 | g | 5 | 59.519 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Vitamin B12 | 10.0 | µg | 1.0e-5 | 7.4e-6 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 9.0-9.5 | |||||
| 2 Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere. | |||||
| 3 Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S x 9 H2O, Na2CO3 from sterile stock solutions. The final pH of the medium should be 9.0-9.5. | |||||
Media with this solution
| 1369 | ECTOTHIORHODOSPIRA MAGNA MEDIUM |