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Main sol. 1370

Internal Solution-ID: S2764

Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.3 g 0.3 1.217
KH2PO4 0.3 g 0.3 2.205
NH4Cl 0.1 g 0.1 1.87
CaCl2 x 2 H2O 1.0 g 1 6.802
Na-acetate 1.0 g 1 12.19
Yeast extract 0.5 g 0.5 -
Peptone 0.5 g 0.5 -
Casamino acids 0.5 g 0.5 -
Distilled water 1000.0 ml - -
1 Adjust pH to 5.9. Add NaOH (0.1 mol/l) 16ml/l after sterilisation from sterile stock solution to readjust pH to 6.8.
2 Add the following from sterile stock solutions to the autoclaved medium:
Trace elements solution N1 2.0 ml - -
Vitamin solution N1 2.0 ml - -
1370 RICH ORGANIC MEDIUM