Main sol. 1370
Internal Solution-ID: S2764
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 0.3 | g | 0.3 | 1.217 | |
KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
NH4Cl | 0.1 | g | 0.1 | 1.87 | |
CaCl2 x 2 H2O | 1.0 | g | 1 | 6.802 | |
Na-acetate | 1.0 | g | 1 | 12.19 | |
Yeast extract | 0.5 | g | 0.5 | - | |
Peptone | 0.5 | g | 0.5 | - | |
Casamino acids | 0.5 | g | 0.5 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust pH to 5.9. Add NaOH (0.1 mol/l) 16ml/l after sterilisation from sterile stock solution to readjust pH to 6.8. | |||||
2 Add the following from sterile stock solutions to the autoclaved medium: | |||||
Trace elements solution N1 | 2.0 | ml | - | - | |
Vitamin solution N1 | 2.0 | ml | - | - |
1370 | RICH ORGANIC MEDIUM |