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DSMZ

DSMZ 1370 . RICH ORGANIC MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1370 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.3 g 0.3 1.217
KH2PO4 0.3 g 0.3 2.205
NH4Cl 0.1 g 0.1 1.87
CaCl2 x 2 H2O 1.0 g 1 6.802
Na-acetate 1.0 g 1 12.19
Yeast extract 0.5 g 0.5 -
Peptone 0.5 g 0.5 -
Casamino acids 0.5 g 0.5 -
Distilled water 1000.0 ml - -
1 Adjust pH to 5.9. Add NaOH (0.1 mol/l) 16ml/l after sterilisation from sterile stock solution to readjust pH to 6.8.
2 Add the following from sterile stock solutions to the autoclaved medium:
Trace elements solution N1 2.0 ml - -
Vitamin solution N1 2.0 ml - -

Vitamin solution N1 #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 400 mg 0.4 3.249
Thiamine 400 mg 0.4 1.507
Biotin 80 mg 0.08 0.327
Vitamin B12 20 mg 0.02 0.015
Distilled water 1000 ml - -
1 Filter sterilised

Trace elements solution N1 #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 300 mg 0.3 1.079
ZnSO4 x 7 H2O 5 mg 0.005 0.017
MnCl2 x 4 H2O 3 mg 0.003 0.015
H3BO3 2 mg 0.002 0.032
CoCl2 x 6 H2O 5 mg 0.005 0.039
CuCl2 x 2 H2O 1 mg 1.0e-3 0.006
NiCl2 x 6 H2O 2 mg 0.002 0.015
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-acetate 1 12.19
CaCl2 x 2 H2O 1 6.802
Peptone 0.5 -
Casamino acids 0.5 -
Yeast extract 0.5 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain