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Main sol. 1382

Internal Solution-ID: S2802

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.10 g 0.1 0.735
CaCl2 x 2 H2O 0.02 g 0.02 0.136
NH4Cl 0.20 g 0.2 3.739
Yeast extract 0.10 g 0.1 -
Glucose 0.50 g 0.5 2.775
Trace element solution (SLA) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 5.0-6.5
2 Serum flasks are filled with Medium by 5/6 of the total volume.
3 Sterilize at 121°C for 15 minutes.
1382 MP MEDIUM