1382
1382 .
MP MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1382 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
| NH4Cl | 0.20 | g | 0.2 | 3.739 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Glucose | 0.50 | g | 0.5 | 2.775 | |
| Trace element solution (SLA) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 5.0-6.5 | |||||
| 2 Hungate tubes are filled with medium by 2/3 of the total volume (e.g., 10 ml in 15 ml tubes). | |||||
| 3 Sterilize at 121°C for 15 minutes. | |||||
Solution occurs in 4
other media (first defined in Medium 351).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeCl2 x 4 H2O | 1.8 | g | 1.8 | 9.054 | |
| CoCl2 x 6 H2O | 250.0 | mg | 0.25 | 1.925 | |
| NiCl2 x 6 H2O | 10.0 | mg | 0.01 | 0.077 | |
| CuCl2 x 2 H2O | 10.0 | mg | 0.01 | 0.059 | |
| MnCl2 x 4 H2O | 70.0 | mg | 0.07 | 0.354 | |
| ZnCl2 | 100.0 | mg | 0.1 | 0.734 | |
| H3BO3 | 500.0 | mg | 0.5 | 8.085 | |
| Na2MoO4 x 2 H2O | 30.0 | mg | 0.03 | 0.124 | |
| Na2SeO3 x 5 H2O | 10.0 | mg | 0.01 | 0.038 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 2.0 - 3.0. | |||||
Metadata
| Last modified: | 05.06.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.0 - 6.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 0.5 | 2.775 |
| NH4Cl | 0.2 | 3.739 |
| KH2PO4 | 0.1 | 0.735 |
| Yeast extract | 0.1 | - |
| CaCl2 x 2 H2O | 0.02 | 0.136 |
| Show more | ||
