Main sol. 1390
Internal Solution-ID: S2820
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.1 | g | 0.1 | - | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| Na2SO4 | 0.7 | g | 0.7 | 4.928 | |
| NaCl | 120.0 | g | 120 | 2053.39 | |
| NaHCO3 | 3.0 | g | 3 | 35.711 | |
| Na2S x 9 H2O | 0.2 | g | 0.2 | 0.833 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Na-ascorbate | 0.5 | g | 0.5 | 2.524 | |
| Na-malate OR Na-acetate | 1.0 | g | 1 | 5.616 | |
| Trace element solution (SLA) | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Vitamin solution (VA) | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust ph to 7.0 | |||||
| 2 Prepare the medium under N2 gas without the NaHCO3, Na2S x 9 H2O, VA-Vitamin solution and vitamin B12. Autoclave and add the VA-Vitamin solution and vitamin B12 from a filter-sterilised stock solution and the NaHCO3, Na2S x 9 H2O, from sterile stock solutions. The final pH of the medium should be 7.0. | |||||
Media with this solution
| 1390 | RHODOVULUM MEDIUM |