1390
1390 .
RHODOVULUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1390 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.1 | g | 0.1 | - | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| Na2SO4 | 0.7 | g | 0.7 | 4.928 | |
| NaCl | 120.0 | g | 120 | 2053.39 | |
| NaHCO3 | 3.0 | g | 3 | 35.711 | |
| Na2S x 9 H2O | 0.2 | g | 0.2 | 0.833 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Na-ascorbate | 0.5 | g | 0.5 | 2.524 | |
| Na-malate OR Na-acetate | 1.0 | g | 1 | 5.616 | |
| Trace element solution (SLA) | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Vitamin solution (VA) | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust ph to 7.0 | |||||
| 2 Prepare the medium under N2 gas without the NaHCO3, Na2S x 9 H2O, VA-Vitamin solution and vitamin B12. Autoclave and add the VA-Vitamin solution and vitamin B12 from a filter-sterilised stock solution and the NaHCO3, Na2S x 9 H2O, from sterile stock solutions. The final pH of the medium should be 7.0. | |||||
Solution occurs in 4
other media (first defined in Medium 351).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeCl2 x 4 H2O | 1.8 | g | 1.8 | 9.054 | |
| CoCl2 x 6 H2O | 250.0 | mg | 0.25 | 1.925 | |
| NiCl2 x 6 H2O | 10.0 | mg | 0.01 | 0.077 | |
| CuCl2 x 2 H2O | 10.0 | mg | 0.01 | 0.059 | |
| MnCl2 x 4 H2O | 70.0 | mg | 0.07 | 0.354 | |
| ZnCl2 | 100.0 | mg | 0.1 | 0.734 | |
| H3BO3 | 500.0 | mg | 0.5 | 8.085 | |
| Na2MoO4 x 2 H2O | 30.0 | mg | 0.03 | 0.124 | |
| Na2SeO3 x 5 H2O | 10.0 | mg | 0.01 | 0.038 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 2.0 - 3.0. | |||||
Solution occurs in 3
other media (first defined in Medium 351).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 10 | mg | 0.1 | 0.409 |
| Nicotinic acid amide | 35 | mg | 0.35 | 2.866 |
| Thiamine-HCl x 2 H2O | 30 | mg | 0.3 | 0.804 |
| p-Aminobenzoic acid | 20 | mg | 0.2 | 1.458 |
| Pyridoxal hydrochloride | 10 | mg | 0.1 | 0.491 |
| Calcium pantothenate | 10 | mg | 0.1 | 0.21 |
| Vitamin B12 | 5 | mg | 0.05 | 0.037 |
| Distilled water | 100 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |