Main sol. 1414
Internal Solution-ID: S2879
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 2.0 | g | 2 | 8.114 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| KCl | 0.3 | g | 0.3 | 4.024 | |
| Na2SO4 | 15.0 | g | 15 | 105.604 | |
| Na acetate | 1.0 | g | 1 | 12.19 | |
| Malic acid | 1.0 | g | 1 | 7.458 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Bacto peptone | 0.5 | g | 0.5 | - | |
| Trace Element Solution | 2.0 | ml | - | - | |
| Vitamine Solution | 2.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust to pH 5.9 before autoclaving | |||||
| 2 Prepare the liquid medium. Before inoculation add from sterile stock solutions: | |||||
|
NaHCO3
(10% w/v) |
5.0 | ml/l | 0.5 | 5.952 | |
|
CaCl2
(10% w/v) |
20.0 | ml/l | 2 | 18.021 | |
|
KH2PO4
(10% w/v) |
3.0 | ml/l | 0.3 | 2.205 | |
| Vitamine Solution | 2.0 | ml/l | - | - | |
| 3 Adjust to pH 8.0 - 8.5 with 0.5 N NaOH. | |||||
Media with this solution
| 1414 | PORPHYROBACTER MEDIUM |