1414
1414 .
PORPHYROBACTER MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1414 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 2.0 | g | 2 | 8.114 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| KCl | 0.3 | g | 0.3 | 4.024 | |
| Na2SO4 | 15.0 | g | 15 | 105.604 | |
| Na acetate | 1.0 | g | 1 | 12.19 | |
| Malic acid | 1.0 | g | 1 | 7.458 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Bacto peptone | 0.5 | g | 0.5 | - | |
| Trace Element Solution | 2.0 | ml | - | - | |
| Vitamine Solution | 2.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust to pH 5.9 before autoclaving | |||||
| 2 Prepare the liquid medium. Before inoculation add from sterile stock solutions: | |||||
|
NaHCO3
(10% w/v) |
5.0 | ml/l | 0.5 | 5.952 | |
|
CaCl2
(10% w/v) |
20.0 | ml/l | 2 | 18.021 | |
|
KH2PO4
(10% w/v) |
3.0 | ml/l | 0.3 | 2.205 | |
| Vitamine Solution | 2.0 | ml/l | - | - | |
| 3 Adjust to pH 8.0 - 8.5 with 0.5 N NaOH. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeSO4 x 7 H2O | 300 | mg | 0.3 | 1.079 | |
| MnCl2 x 4 H2O | 3 | mg | 0.003 | 0.015 | |
| CoCl2 x 6 H2O | 5 | mg | 0.005 | 0.039 | |
| CuCl2 x 2 H2O | 1 | mg | 1.0e-3 | 0.006 | |
| NiCl2 x 6 H2O | 2 | mg | 0.002 | 0.015 | |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 | |
| ZnSO4 x 7 H2O | 5 | mg | 0.005 | 0.017 | |
| H3BO3 | 2 | mg | 0.002 | 0.032 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust to pH 3.0 | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nicotinic acid | 400 | mg | 0.4 | 3.249 | |
| Thiamine | 400 | mg | 0.4 | 1.507 | |
| Biotine | 80 | mg | 0.08 | 0.327 | |
| Vitamin B12 | 20 | mg | 0.02 | 0.015 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Filtersterilized | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 8.0 - 8.5 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2SO4 | 15 | 105.604 |
| CaCl2 | 2 | 18.021 |
| MgSO4 x 7 H2O | 2 | 8.114 |
| Na acetate | 1 | 12.19 |
| Malic acid | 1 | 7.458 |
| Show more | ||
