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Solution 1

Internal Solution-ID: S2943

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.25 g 0.263 -
Yeast extract 0.25 g 0.263 -
CaCO3 5.00 g 5.263 52.584
Na2HPO4 x H2O 0.10 g 0.105 0.742
MgSO4 x 7 H2O 0.50 g 0.526 2.135
Hutner's salts 20.00 ml - -
Gelrite 9.00 g 9.474 -
Distilled water 930.00 ml - -
1 Adjust pH to 9.0 with NaOH, autoclave at 121°C for 20 min.
1437 ALKALIPHILES PIRELLULA MEDIUM (M1PY)