Solution 1
Internal Solution-ID: S2947
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 0.25 | g | 0.263 | - | |
Yeast extract | 0.25 | g | 0.263 | - | |
CaCl2 x 2 H2O | 0.10 | g | 0.105 | 0.716 | |
MgCl2 x 6 H2O | 0.10 | g | 0.105 | 0.518 | |
Hutner's salts | 20.00 | ml | - | - | |
Tris-HCl buffer
(0.1M, pH 7.5) |
50.00 | ml | - | 5 | |
Agar | 18.00 | g | 18.947 | 56.336 | |
Distilled water | 880.00 | ml | - | - | |
1 Adjust pH to 7.5, autoclave at 121°C for 20 min. | |||||
2 For agar plates increase the pH of the Tris/HCl buffer to 8.5. |
1438 | PIRELLULA MEDIUM (M31 PY) |