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Solution 1

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Internal Solution-ID: S2951

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.25 g 0.26 -
Yeast extract 0.25 g 0.26 -
CaCl2 0.20 g 0.208 1.877
Na2HPO4 x H2O 0.10 g 0.104 0.734
MgSO4 x 7 H2O 0.50 g 0.521 2.113
Hutner's salts 20.00 ml - -
Gelrite 9.00 g 9.375 -
Distilled water 940.00 ml - -
1 Adjust pH to 9.0, autoclave at 121°C for 20 min.
1439 ALKALIPHILES PIRELLULA MEDIUM (M1aPY)