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Main sol. 1000

Internal Solution-ID: S3

Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.000 g 2.997 51.281
2 0.014 g 0.014 0.08
3 0.014 g 0.014 0.095
4 0.040 g 0.04 0.747
5 0.340 g 0.34 1.378
6 0.418 g 0.418 2.054
7 0.033 g 0.033 0.442
8 0.005 g 0.005 -
9 0.005 mg 5.0e-6 2.1e-5
10 0.005 mg 5.0e-6 1.9e-5
11 0.500 g 0.5 1.758
12 Trace mineral solution 1.000 ml - -
13 1000.000 ml 997.21 55353.5
14 10.000 mM 2.482 10
15 0.200 % 2 23.808
16 0.050 % 0.5 5.883
17 , required for pH adjustment x - -
Instructions:
  1. Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below (#14-16). Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm.
1000 MJANHOX-NO3 MEDIUM WITH SUPPLEMENT