Main sol. 1479
Internal Solution-ID: S3029
Equipment needed: Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 240.0 | g | 240 | 4106.78 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| (NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
| HEPES | 10.0 | g | 10 | 41.964 | |
| Trace element solution | 1.0 | ml | - | - | |
| Sulfur powder | 2.0 | g | 2 | 7.797 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions: | |||||
| MgCl2 x 6 H2O | 3.0 | mM | 0.61 | 3 | |
| NaHCO3 | 5.0 | mM | 0.42 | 5 | |
| Sodium formate | 50.0 | mM | 3.4 | 50 | |
| Yeast extract | 0.2 | g/l | 0.2 | - | |
| Vitamin mix (pfennig & Lippert,1966) | 1.0 | ml | - | - | |
|
Disodium sulfide
(1M filter sterilized stock) |
1.0 | mM | - | 1 | |
Media with this solution
| 1479 | HALODESULFURIARCHAEUM MEDIUM |