Main sol. 182
Internal Solution-ID: S308
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
|
Casamino acids
(BD BBL) |
1.00 | g | 1 | - | |
| KH2PO4 | 3.10 | g | 3.1 | 22.78 | |
| (NH4)2SO4 | 2.50 | g | 2.5 | 18.919 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
| Allen's trace element solution | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve all compounds except yeast extract and Casamino acids, adjust pH to 4.0 - 4.2 with 10 N H2SO4 at room temperature and autoclave. Prior to inoculation add yeast extract and Casamino acids from stock solutions sterilized by filtration. | |||||
Media with this solution
| 182 | SACCHAROLOBUS SOLFATARICUS MEDIUM |