Main sol. 1511
Internal Solution-ID: S3121
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.33 | g | 0.33 | 2.425 | |
NH4Cl | 0.33 | g | 0.33 | 6.169 | |
MgCl2 | 1.20 | mg | 0.001 | 0.013 | |
CaCl2 | 1.20 | mg | 0.001 | 0.011 | |
KCl | 0.33 | g | 0.33 | 4.427 | |
Trace Element Solution | 1.00 | ml | - | - | |
Wolin's vitamin solution | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: | |||||
KNO3 | 1.00 | g | 1 | 9.891 | |
Glucose | 2.00 | g | 2 | 11.101 | |
Wolin's vitamin solution | 1.00 | ml | - | - | |
NaHCO3
(8% solution) |
12.50 | ml | 1 | 11.904 | |
2 The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume. | |||||
3 The final pH of the medium should be adjusted to pH 7.6-8.0. |
1511 | PLANTOMYCETACEAE MEDIUM SBP2 |