This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.33 | g | 0.33 | 2.425 | |
NH4Cl | 0.33 | g | 0.33 | 6.169 | |
MgCl2 | 1.20 | mg | 0.001 | 0.013 | |
CaCl2 | 1.20 | mg | 0.001 | 0.011 | |
KCl | 0.33 | g | 0.33 | 4.427 | |
Trace Element Solution | 1.00 | ml | - | - | |
Wolin's vitamin solution | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: | |||||
KNO3 | 1.00 | g | 1 | 9.891 | |
Glucose | 2.00 | g | 2 | 11.101 | |
Wolin's vitamin solution | 1.00 | ml | - | - | |
NaHCO3
(8% solution) |
12.50 | ml | 1 | 11.904 | |
2 The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume. | |||||
3 The final pH of the medium should be adjusted to pH 7.6-8.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Fe(NH4)2(SO4)2 x 6 H2O | 784 | mg | 0.784 | - |
NiCl2 x 2 H2O | 284 | mg | 0.284 | 2.191 |
CoCl2 x 6 H2O | 38 | mg | 0.038 | 0.293 |
MnCl2 x 4 H2O | 99 | mg | 0.099 | 0.5 |
ZnSO4 x 7 H2O | 144 | mg | 0.144 | 0.501 |
Na2SeO4 | 95 | mg | 0.095 | 0.503 |
Na2WO4 x 2 H2O | 33 | mg | 0.033 | 0.1 |
Na2MoO4 x 2 H2O | 24 | mg | 0.024 | 0.099 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Medium type: | Defined medium |
Final pH: | 7.6 - 8.0 |
Equipment needed: | Hungate tubes |
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