DSMZ 1511 . PLANTOMYCETACEAE MEDIUM SBP2

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.33 g 0.33 2.425
NH4Cl 0.33 g 0.33 6.169
MgCl2 1.20 mg 0.001 0.013
CaCl2 1.20 mg 0.001 0.011
KCl 0.33 g 0.33 4.427
Trace Element Solution 1.00 ml - -
Wolin's vitamin solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
KNO3 1.00 g 1 9.891
Glucose 2.00 g 2 11.101
Wolin's vitamin solution 1.00 ml - -
NaHCO3
(8% solution)
12.50 ml 1 11.904
2 The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume.
3 The final pH of the medium should be adjusted to pH 7.6-8.0.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 1 other media (first defined in Medium 1473).
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 784 mg 0.784 -
NiCl2 x 2 H2O 284 mg 0.284 2.191
CoCl2 x 6 H2O 38 mg 0.038 0.293
MnCl2 x 4 H2O 99 mg 0.099 0.5
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2SeO4 95 mg 0.095 0.503
Na2WO4 x 2 H2O 33 mg 0.033 0.1
Na2MoO4 x 2 H2O 24 mg 0.024 0.099
H3BO3 6 mg 0.006 0.097
CuCl2 x 2 H2O 2 mg 0.002 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.6 - 8.0
Equipment needed: Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 2 11.101
NaHCO3 1 11.904
KNO3 1 9.891
NH4Cl 0.33 6.169
KCl 0.33 4.427
Show more