Main sol. 1564
Internal Solution-ID: S3236
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 1566 | 994 | ml | - | - | |
| 1 Autoclave at 121 °C for 20 min. | |||||
| 2 Before inoculation add from sterile stock solutions: | |||||
|
NaHCO3
(1M in H2O) |
2 | ml | 0.17 | 2.024 | |
|
α-D-Glucose monohydrate
(10% in H2O) |
1 | ml | 0.4 | 2.22 | |
| Selenite-tungstate solution | 2 | ml | - | - | |
|
Cellobiose
(10% in H2O) |
1 | ml | 0.4 | 2.22 | |
| 3 Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3 | |||||
Media with this solution
| 1564 | HAHA100-MEDIUM |