Main sol. 1566
Internal Solution-ID: S3240
This solution is used in 2 media and was first defined in medium 1566.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.37 | g | - | - | |
| MgSO4 x 7 H2O | 6.80 | g | - | - | |
| MgCl2 x 6 H2O | 5.67 | g | - | - | |
| CaCl2 x 2 H2O | 1.47 | g | - | - | |
| KCl | 0.72 | g | - | - | |
| KBr | 0.10 | g | - | - | |
| NH4Cl | 0.80 | mg | - | - | |
| KH2PO4 | 0.02 | g | - | - | |
| H3BO3 | 0.02 | g | - | - | |
| SrCl2 | 0.02 | g | - | - | |
| NaF | 3.00 | mg | - | - | |
| Tryptone peptone | 0.10 | g | - | - | |
|
Casamino acids
(Oxoid) |
0.10 | g | - | - | |
| Yeast extract | 0.10 | g | - | - | |
|
Micronutrient solution SL 8
(medium 1162) |
2.00 | ml | - | - | |
| Distilled water | 982.00 | ml | - | - | |
| 1 Autoclave at 121 °C for 20 min. | |||||
| 2 Before inoculation add from sterile stock solutions: | |||||
|
α-D-Glucose monohydrate
(10% in H2O) |
1.00 | ml | - | - | |
|
Cellobiose
(10% in H2O) |
1.00 | ml | - | - | |
| Standard vitamin solution | 10.00 | ml | - | - | |
|
NaHCO3
(1M in H2O) |
2.00 | ml | - | - | |
| Selenite-tungstate solution | 2.00 | ml | - | - | |
| 3 Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3 | |||||