1564
1564 .
HAHA100-MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1564 #
ml
Equipment needed: Autoclave
| 1 Before inoculation add from sterile stock solutions: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 1566 | 994 | ml | - | - | |
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3 | |||||
|
α-D-Glucose monohydrate
(10% in H2O) |
1 | ml | 0.4 | 2.22 | |
|
Cellobiose
(10% in H2O) |
1 | ml | 0.4 | 2.22 | |
|
NaHCO3
(1M in H2O) |
2 | ml | 0.17 | 2.024 | |
| Selenite-tungstate solution | 2 | ml | - | - | |
Solution occurs in 2
other media (first defined in Medium 1162).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 5.2 | g | - | - |
| FeCl2 x 4 H2O | 1.5 | g | - | - |
| ZnCl2 | 70.0 | mg | - | - |
| MnCl2 x 4 H2O | 100.0 | mg | - | - |
| H3BO3 | 62.0 | mg | - | - |
| CoCl2 x 6 H2O | 190.0 | mg | - | - |
| CuCl2 x 2 H2O | 17.0 | mg | - | - |
| NiCl2 x 6 H2O | 24.0 | mg | - | - |
| Na2MoO4 x 2 H2O | 36.0 | mg | - | - |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium 1566).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.37 | g | - | - | |
| MgSO4 x 7 H2O | 6.80 | g | - | - | |
| MgCl2 x 6 H2O | 5.67 | g | - | - | |
| CaCl2 x 2 H2O | 1.47 | g | - | - | |
| KCl | 0.72 | g | - | - | |
| KBr | 0.10 | g | - | - | |
| NH4Cl | 0.80 | mg | - | - | |
| KH2PO4 | 0.02 | g | - | - | |
| H3BO3 | 0.02 | g | - | - | |
| SrCl2 | 0.02 | g | - | - | |
| NaF | 3.00 | mg | - | - | |
| Tryptone peptone | 0.10 | g | - | - | |
|
Casamino acids
(Oxoid) |
0.10 | g | - | - | |
| Yeast extract | 0.10 | g | - | - | |
|
Micronutrient solution SL 8
(medium 1162) |
2.00 | ml | - | - | |
| Distilled water | 982.00 | ml | - | - | |
| 1 Autoclave at 121 °C for 20 min. | |||||
| 2 Before inoculation add from sterile stock solutions: | |||||
|
α-D-Glucose monohydrate
(10% in H2O) |
1.00 | ml | - | - | |
|
Cellobiose
(10% in H2O) |
1.00 | ml | - | - | |
| Standard vitamin solution | 10.00 | ml | - | - | |
|
NaHCO3
(1M in H2O) |
2.00 | ml | - | - | |
| Selenite-tungstate solution | 2.00 | ml | - | - | |
| 3 Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3 | |||||
Solution occurs in 178
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Synonyms: | HAHA100MOD-MEDIUM |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 26.4 | 451.745 |
| MgSO4 x 7 H2O | 6.8 | 27.589 |
| MgCl2 x 6 H2O | 5.7 | 28.037 |
| CaCl2 x 2 H2O | 1.5 | 10.203 |
| KCl | 0.7 | 9.39 |
| Show more | ||
