Main sol. 1565
Internal Solution-ID: S3238
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 100.00 | ml | - | - | |
|
Yeast extract
(BD Bacto) |
2.00 | g | 2 | - | |
|
Tryptone
(BD Bacto) |
2.00 | g | 2 | - | |
| Na2-tartrate x 2 H2O | 1.00 | g | 1 | - | |
| Na3-citrate | 1.00 | g | 1 | 3.875 | |
| NaNO3 | 0.25 | g | 0.25 | 2.941 | |
| (NH4)2SO4 | 0.25 | g | 0.25 | 1.892 | |
| Ferric citrate monohydrate | 0.20 | g | 0.2 | 0.761 | |
| Na2SO4 | 1.00 | g | 1 | 7.04 | |
| Na2CO3 | 1.25 | g | 1.25 | 11.794 | |
| NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, bicarbonate, and thiosulfate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and bicarbonate, adjust pH to 10 with KOH, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate to the autoclaved medium from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust the pH of the complete medium to 9.8 - 10.0. | |||||
Media with this solution
| 1565 | ALKALIPHILUS TRANSVAALENSIS MEDIUM |