MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1585a

Download this solution as

CSV JSON PDF

Internal Solution-ID: S3276

Equipment needed: Serum bottle Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 0.2 g 0.2 0.984
CaCl2 x 2 H2O 0.1 g 0.1 0.68
(NH4)2SO4 0.1 g 0.1 0.757
KH2PO4 0.1 g 0.1 0.735
KCl 0.1 g 0.1 1.341
MOPS
(SIGMA M1254) 		4.2 g 4.2 20.071
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Na2S2O3 x 5 H2O 5.0 g 5 20.146
NaHCO3 2.5 g 2.5 29.759
Distilled water 1000.0 ml - -
1 Dissolve ingredients except thiosulfate and bicarbonate, then adjust pH of medium to 6.5. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate and bicarbonate added from stock solutions sterilized by filtration.
1585a SULFURIFERULA THIOPHILA MEDIUM