1585a
1585a .
SULFURIFERULA THIOPHILA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1585a #
ml
Equipment needed: Serum bottle
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| (NH4)2SO4 | 0.1 | g | 0.1 | 0.757 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.735 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
|
MOPS
(SIGMA M1254) |
4.2 | g | 4.2 | 20.071 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2S2O3 x 5 H2O | 5.0 | g | 5 | 20.146 | |
| NaHCO3 | 2.5 | g | 2.5 | 29.759 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except thiosulfate and bicarbonate, then adjust pH of medium to 6.5. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate and bicarbonate added from stock solutions sterilized by filtration. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 179
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 11.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave, Filter, Serum bottle |
| Edit in Medium builder | |
Gas composition
- 100 % Air
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2S2O3 x 5 H2O | 5 | 20.146 |
| MOPS | 4.2 | 20.071 |
| NaHCO3 | 2.5 | 29.759 |
| MgCl2 x 6 H2O | 0.2 | 0.984 |
| KCl | 0.1 | 1.341 |
| Show more | ||
