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1585a. SULFURIFERULA THIOPHILA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 0.2 0.982
2 0.1 g 0.1 0.679
3 0.1 g 0.1 0.755
4 0.1 g 0.1 0.733
5 0.1 g 0.1 1.339
6
(SIGMA M1254)
4.2 g 4.192 -
7 Trace element solution SL-10 1.0 ml - -
8 Selenite-tungstate solution 1.0 ml - -
9 5.0 g 4.99 20.106
10 2.5 g 2.495 29.7
11 1000.0 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients except thiosulfate and bicarbonate, then adjust pH of medium to 6.5. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate and bicarbonate added from stock solutions sterilized by filtration.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Filter

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
Na2S2O3 x 5 H2O 4.99 20.106
MOPS 4.192 -
NaHCO3 2.495 29.7
MgCl2 x 6 H2O 0.2 0.982
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Cultivation metadata from 2 strains BacDive

halophily not 2 strains
pH 4 - 6 2 strains
pH 6 - 8 2 strains
pH 8 - 10 2 strains
phyla 1 2 strains
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