Main sol. 1712
Internal Solution-ID: S3542
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | 0.526 | 3.868 | |
| NH4Cl | 0.3 | g | 0.316 | 5.904 | |
| MgSO4 x 7 H2O | 0.5 | g | 0.526 | 2.135 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.105 | 0.716 | |
| NaNO3 | 0.6 | g | 0.632 | 7.431 | |
| Distilled water | 950.0 | mL | - | - | |
| 1 The medium was autoclaved and removed from the autoclave at 85°C. Oxygen dissolution was prevented by exchanging the gas-phase with N2. The medium was complemented by addition of the following stock solutions: | |||||
| Trace element solution | 1.0 | mL/L | - | - | |
| Vitamin mixture | 1.0 | mL/L | - | - | |
| Vitamin B12 solution | 1.0 | mL/L | - | - | |
| Bicarbonate solution (1M) | 40.0 | mL/L | 3.36 | 40 | |
| 2 After adding bicarbonate the gas was changed from N2 to N2/CO2 (90/10 %, v/v) and the pH was adjusted to 7.2 - 7.4 with sterile 2 M HCL. | |||||
| Ascorbate solution (1M) | 4.0 | mL/L | - | 4 | |
| Benzoate solution (1M) | 4.0 | mL/L | 0.484 | 4 | |
Media with this solution
| 1712 | AROMATOLEUM MEDIUM |