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Main sol. J66

Internal Solution-ID: S3693

Equipment needed: Centrifuge Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
V8 canned vegetable juice
(Campbell)
200 ml - -
CaCO3 3 g 3 29.973
Agar 15 g 15 44.599
Distilled water 800 ml - -
1 Adjust pH to 7.2.
2 Filter V8 vegetable juice through 4 layers of cheesecloth or centrifuge (3,000 rpm, 10 min) to eliminate fibers.
J66 V8 JUICE AGAR