Main sol. J180
Internal Solution-ID: S3838
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.20 | g | 0.2 | 1.514 | |
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
|
Yeast extract
(BD-Difco) |
1.00 | g | 1 | - | |
| Glucose | 10.00 | g | 10 | 55.506 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 2.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium. | |||||
Media with this solution
| J180 | THERMOPLASMA MEDIUM |