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Main sol. J181

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Internal Solution-ID: S3839

Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 10.0 g 11.111 61.674
Polypeptone
(Nihon Pharm. Co.) 		5.0 g 5.556 -
Yeast extract 5.0 g 5.556 -
K2HPO4 1.0 g 1.111 6.379
MgSO4 x 7 H2O 0.2 g 0.222 0.902
Agar 15.0 g 16.667 49.555
Distilled water 900.0 ml - -
1 After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.
J181 HORIKOSHI-I MEDIUM