J181
181 .
HORIKOSHI-I MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J181 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 10.0 | g | 11.111 | 61.674 | |
|
Polypeptone
(Nihon Pharm. Co.) |
5.0 | g | 5.556 | - | |
| Yeast extract | 5.0 | g | 5.556 | - | |
| K2HPO4 | 1.0 | g | 1.111 | 6.379 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.222 | 0.902 | |
| Agar | 15.0 | g | 16.667 | 49.555 | |
| Distilled water | 900.0 | ml | - | - | |
| 1 After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 10.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 52 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 16.667 | 49.555 |
| Glucose | 11.111 | 61.674 |
| Polypeptone | 5.556 | - |
| Yeast extract | 5.556 | - |
| K2HPO4 | 1.111 | 6.379 |
| Show more | ||
