Main sol. J215
Internal Solution-ID: S3881
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Marine medium/Synthetic seawater mix solution | 125.00 | ml | - | - | |
| Trace minerals | 10.00 | ml | - | - | |
| Fe(NH4)2(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | - | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | 0.005 | |
| Na2SeO4 | 0.10 | mg | 1.0e-4 | 5.3e-4 | |
| Na2WO4 x 2 H2O | 0.10 | mg | 1.0e-4 | - | |
| NaHCO3 | 2.20 | g | 2.2 | 26.188 | |
| KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
|
Yeast extract
(BD-Difco) |
0.50 | g | 0.5 | - | |
| Na2S2O3 x 5 H2O | 1.00 | g | 1 | 4.029 | |
| Distilled water | 865.00 | ml | - | - | |
| 1 Adjust pH to 7.0 with 1 N H2SO4, and sterilize by filtration. Dispense the medium into bottles under a N2-CO2-O2 (80:20:1) atmosphere. Pressurize inoculated bottles to 200 kPa N2-CO2-O2 (80:20:1). | |||||
Media with this solution
| J215 | PYROBACULUM AEROPHILUM MEDIUM |