Main sol. J279
Internal Solution-ID: S3971
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 7.00 | g | 6.931 | 28.119 | |
| MgCl2 x 6 H2O | 5.50 | g | 5.446 | 26.785 | |
| KCl | 0.65 | g | 0.644 | 8.633 | |
| NaBr | 0.10 | g | 0.099 | 0.962 | |
| NaHCO3 | 2.00 | g | 1.98 | 23.572 | |
| NH4Cl | 0.15 | g | 0.149 | 2.777 | |
| CaCl2 x 2 H2O | 0.50 | g | 0.495 | 3.367 | |
| Trace minerals | 10.00 | ml | - | - | |
| (NH4)2Ni(SO4)2 x 6 H2O | 0.02 | g | 0.02 | 0.05 | |
| Na2WO4 x 2 H2O | 0.10 | mg | 9.9e-5 | 3.0e-4 | |
| Na2SeO4 | 0.10 | mg | 9.9e-5 | 5.2e-4 | |
| Na2S2O3 x 5 H2O | 1.00 | g | 0.99 | 3.989 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0 with H2SO4. Distribute 20 ml of medium into 120 ml serum bottles under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Autoclave and stand the bottles overnight. Add 15 ml filter-sterilized air and pressurize the gas phase to 200 kPa with a H2-CO2 (4:1, v/v) gas mixture. | |||||
Media with this solution
| J279 | HYDROGENOPHILUS HIRSCHII MEDIUM |