Main sol. J358
Internal Solution-ID: S4077
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trace element solution SLA | 1.0 | ml | - | - | |
| Vitamin solution VA | 1.0 | ml | - | - | |
| KH2PO4 | 1.0 | g | 0.998 | 7.334 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.499 | 2.454 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| NaCl | 1.0 | g | 0.998 | 17.077 | |
| Sodium acetate | 1.0 | g | 0.998 | 12.166 | |
| Sodium succinate | 1.0 | g | 0.998 | 6.159 | |
|
Yeast extract
(BD-Difco) |
0.5 | g | 0.499 | - | |
|
Bacto peptone
(BD-Difco) |
0.3 | g | 0.299 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.9. | |||||
| 2 Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium. | |||||
Media with this solution
| J358 | ATYP MEDIUM |