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Main sol. J358

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Internal Solution-ID: S4077

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SLA 1.0 ml - -
Vitamin solution VA 1.0 ml - -
KH2PO4 1.0 g 0.998 7.334
MgCl2 x 6 H2O 0.5 g 0.499 2.454
CaCl2 x 2 H2O 0.1 g 0.1 0.679
NH4Cl 1.0 g 0.998 18.658
NaCl 1.0 g 0.998 17.077
Sodium acetate 1.0 g 0.998 12.166
Sodium succinate 1.0 g 0.998 6.159
Yeast extract
(BD-Difco) 		0.5 g 0.499 -
Bacto peptone
(BD-Difco) 		0.3 g 0.299 -
Distilled water 1000.0 ml - -
1 Adjust pH to 6.9.
2 Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium.
J358 ATYP MEDIUM