J358
358 .
ATYP MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J358 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trace element solution SLA | 1.0 | ml | - | - | |
| Vitamin solution VA | 1.0 | ml | - | - | |
| KH2PO4 | 1.0 | g | 0.998 | 7.334 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.499 | 2.454 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| NaCl | 1.0 | g | 0.998 | 17.077 | |
| Sodium acetate | 1.0 | g | 0.998 | 12.166 | |
| Sodium succinate | 1.0 | g | 0.998 | 6.159 | |
|
Yeast extract
(BD-Difco) |
0.5 | g | 0.499 | - | |
|
Bacto peptone
(BD-Difco) |
0.3 | g | 0.299 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.9. | |||||
| 2 Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeCl2 x 4 H2O | 1800 | mg | 1.8 | 9.054 | |
| CoCl2 x 6 H2O | 250 | mg | 0.25 | 1.925 | |
| NiCl2 x 6 H2O | 10 | mg | 0.01 | 0.077 | |
| CuCl2 x 2 H2O | 10 | mg | 0.01 | 0.059 | |
| MnCl2 x 4 H2O | 70 | mg | 0.07 | - | |
| ZnCl2 | 100 | mg | 0.1 | 0.734 | |
| H3BO3 | 500 | mg | 0.5 | 8.085 | |
| Na2MoO4 x 2 H2O | 30 | mg | 0.03 | 0.124 | |
| Na2SeO3 x 5 H2O | 10 | mg | 0.01 | 0.038 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 2 - 3 with HCl. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 10 | mg | 0.1 | 0.409 |
| Niacin | 35 | mg | 0.35 | 2.843 |
| Thiamine HCl | 30 | mg | 0.3 | - |
| p-Aminobenzoic acid | 20 | mg | 0.2 | 1.458 |
| Pyridoxine hydrochloride | 10 | mg | 0.1 | - |
| Calcium pantothenate | 10 | mg | 0.1 | 0.21 |
| Vitamin B12 | 5 | mg | 0.05 | 0.037 |
| Distilled water | 100 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.9 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 7 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NH4Cl | 0.998 | 18.658 |
| NaCl | 0.998 | 17.077 |
| Sodium acetate | 0.998 | 12.166 |
| KH2PO4 | 0.998 | 7.334 |
| Sodium succinate | 0.998 | 6.159 |
| Show more | ||
