Main sol. J360
Internal Solution-ID: S4081
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.703 | 508.264 | |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 | |
| CaCl2 x 2 H2O | 1.00 | g | 0.99 | 6.735 | |
| NaNO3 | 0.80 | g | 0.792 | 9.319 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 | |
| MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 | |
| KCl | 0.33 | g | 0.327 | 4.383 | |
| NiCl2 x 6 H2O | 5.00 | mg | 0.005 | 0.038 | |
| Na2SeO3 x 5 H2O | 5.00 | mg | 0.005 | 0.019 | |
| Fe(SO4)3 x n H2O | 0.05 | g | 0.05 | - | |
| H2WO4 | 5.00 | mg | 0.005 | 0.02 | |
| Trace mineral solution | 10.00 | ml | - | - | |
| NaHCO3 | 1.00 | g | 0.99 | 11.786 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | - | |
| Na2S x 9 H2O | 0.50 | g | 0.495 | 2.061 | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, adjust pH to 7.5 - 8.0, and autoclave. Separately autoclave NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O solutions (5% each) under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O solutions aseptically and anaerobically. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v). | |||||
Media with this solution
| J360 | MMJ MEDIUM |