Main sol. J366
Internal Solution-ID: S4087
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.33 | g | 0.324 | 6.048 | |
| KCl | 0.33 | g | 0.324 | 4.34 | |
| KH2PO4 | 0.33 | g | 0.324 | 2.377 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.324 | 2.201 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.324 | 1.591 | |
| NaCl | 30.00 | g | 29.412 | 503.281 | |
|
Yeast extract
(BD-Difco) |
0.30 | g | 0.294 | - | |
| Sodium pyruvate | 2.00 | g | 1.961 | 17.819 | |
| NaNO3 | 1.00 | g | 0.98 | 11.535 | |
| Trace minerals | 10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients except CaCl2 x 2H2O and MgCl2 x 6H2O, adjust pH to 6.5, and autoclave. Separately autoclave CaCl2 x 2H2O and MgCl2 x 6H2O, and add to the medium. Dispense the medium into culture vessels under a N2 atmosphere, and seal with butyl rubber stoppers. | |||||
Media with this solution
| J366 | VULCANITHERMUS MEDIATLANTICUS MEDIUM |