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J366. VULCANITHERMUS MEDIATLANTICUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.324 6.048
2 0.33 g 0.324 4.34
3 0.33 g 0.324 2.377
4 0.33 g 0.324 2.201
5 0.33 g 0.324 1.591
6 30.00 g 29.412 503.26
7
(BD-Difco)
0.30 g 0.294 -
8 2.00 g 1.961 -
9 1.00 g 0.98 11.535
10 Trace minerals 10.00 ml - -
11 Trace vitamins 10.00 ml - -
12 1000.00 ml 978.634 54322.4
Instructions:
  1. Mix ingredients except CaCl2 x 2H2O and MgCl2 x 6H2O, adjust pH to 6.5, and autoclave. Separately autoclave CaCl2 x 2H2O and MgCl2 x 6H2O, and add to the medium. Dispense the medium into culture vessels under a N2 atmosphere, and seal with butyl rubber stoppers.
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 29.422 503.428
Sodium pyruvate 1.961 -
NaNO3 0.98 11.535
CaCl2 x 2H2O 0.325 2.207
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