Main sol. J368
Internal Solution-ID: S4089
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.674 | 507.762 | |
| K2HPO4 | 0.90 | g | 0.89 | 5.111 | |
| KH2PO4 | 0.70 | g | 0.692 | 5.088 | |
| CaCl2 x 2 H2O | 1.00 | g | 0.989 | 6.728 | |
| NH4Cl | 1.25 | g | 1.236 | 23.115 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.363 | 13.644 | |
| MgCl2 x 6 H2O | 4.18 | g | 4.135 | 20.337 | |
| KCl | 0.33 | g | 0.326 | 4.378 | |
| NiCl2 x 6 H2O | 5.00 | mg | 0.005 | 0.038 | |
| Na2SeO3 x 5 H2O | 0.50 | mg | 4.9e-4 | 0.002 | |
| Fe2(SO4)3 x n H2O | 0.01 | mg | 9.9e-6 | - | |
| H2WO4 | 5.00 | mg | 0.005 | 0.02 | |
| FeSO4 x 7 H2O | 0.02 | g | 0.02 | 0.071 | |
| Ferric citrate | 0.02 | g | 0.02 | 0.081 | |
| Trace mineral solution | 10.00 | ml | - | - | |
| Trace vitamins | 1.00 | ml | - | - | |
| NaHCO3 | 1.00 | g | 0.989 | 11.774 | |
|
Sulfur
(powder) |
10.00 | g | 9.891 | 308.425 | |
| Na2S x 9 H2O | 0.50 | g | 0.495 | 2.059 | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S x 9H2O solution. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v). | |||||
Media with this solution
| J368 | MJAIS MEDIUM |