Main sol. J419
Internal Solution-ID: S4153
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 3.000 | g | 2.994 | 51.232 | |
| K2HPO4 | 0.014 | g | 0.014 | 0.08 | |
| CaCl2 x 2 H2O | 0.080 | g | 0.08 | 0.543 | |
| MgSO4 x 7 H2O | 0.340 | g | 0.339 | 1.377 | |
| MgCl2 x 6 H2O | 0.418 | g | 0.417 | 2.052 | |
| KCl | 0.330 | g | 0.329 | 4.418 | |
| NH4Cl | 0.500 | g | 0.499 | 9.329 | |
| Na2S2O3 x 5 H2O | 1.000 | g | 0.998 | 4.021 | |
| Na2SiO3 x 9 H2O | 0.800 | g | 0.798 | - | |
| Fe2(SO4)3 x n H2O | 2.000 | mg | 0.002 | - | |
| FeSO4 x 7 H2O | 0.100 | mg | 1.0e-4 | 3.6e-4 | |
| NiCl2 x 6 H2O | 0.050 | mg | 5.0e-5 | 3.9e-4 | |
| Na2SeO3 x 5 H2O | 0.050 | mg | 5.0e-5 | 1.9e-4 | |
| Trace mineral solution | 1.000 | ml | - | - | |
| Trace vitamins | 1.000 | ml | - | - | |
| NaHCO3 | 0.500 | g | 0.499 | 5.94 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix ingredients, except Trace vitamins and NaHCO3, and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Finally add O2 gas to make 5% (v/v) in the gas phase. | |||||
Media with this solution
| J419 | INORGANIC MINERAL MEDIUM WITH THIOSULFATE |