Main sol. J470
Internal Solution-ID: S4220
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
30.00 | g | 29.354 | - | |
| NaNO3 | 1.00 | g | 0.978 | 11.512 | |
| Wolfe's mineral elixir | 2.00 | ml | - | - | |
| VOSO4 x n H2O | 0.05 | mg | 4.9e-5 | - | |
| Sodium pyruvate | 1.00 | g | 0.978 | 8.892 | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.098 | 0.352 | |
| NaHCO3 | 1.00 | g | 0.978 | 11.648 | |
| Yeast extract | 0.50 | g | 0.489 | - | |
| Trace vitamins | 20.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except FeSO4 x 7H2O, NaHCO3, yeast extract and vitamins, and adjust pH to 7.0. Bring to a boil for 3 min, then cool to room temperature under a N2 gas stream. Dispense the medium under the same gas atmosphere into culture vessels, seal with butyl rubber stoppers and autoclave. Add sterile anaerobic stock solutions of FeSO4 x 7H2O (1%, in 0.1 N H2SO4), NaHCO3 (8%, under a N2 x CO2 gas mixture), yeast extract (10%) and vitamins (filter-sterilized) to the medium. Adjust final pH of the medium to 7.0 - 7.2. Add 10 - 20 mg sodium dithionite per liter [e.g., from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized], if the medium is not completely reduced after inoculation. | |||||
Media with this solution
| J470 | VULCANIBACILLUS MEDIUM |