Main sol. J483
Internal Solution-ID: S4238
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 10.0 | g | 9.804 | 54.418 | |
|
Tryptone
(BD-Difco) |
4.0 | g | 3.922 | 55.171 | |
|
Beef extract
(BD-Difco) |
2.0 | g | 1.961 | - | |
|
Yeast extract
(BD-Difco) |
1.0 | g | 0.98 | - | |
| NaCl | 4.0 | g | 3.922 | 67.104 | |
| K2HPO4 | 1.5 | g | 1.471 | 8.443 | |
| FeSO4 x 7 H2O | 0.1 | g | 0.098 | 0.353 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.196 | 0.964 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.49 | - | |
| Microelement solution | 10.0 | ml | - | - | |
| Vitamin solution | 10.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution. | |||||
Media with this solution
| J483 | ETHANOLOGENBACTERIUM MEDIUM |