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JCM 483 . ETHANOLOGENBACTERIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J483 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 10.0 g 9.804 54.418
Tryptone
(BD-Difco) 		4.0 g 3.922 55.171
Beef extract
(BD-Difco) 		2.0 g 1.961 -
Yeast extract
(BD-Difco) 		1.0 g 0.98 -
NaCl 4.0 g 3.922 67.104
K2HPO4 1.5 g 1.471 8.443
FeSO4 x 7 H2O 0.1 g 0.098 0.353
MgCl2 x 6 H2O 0.2 g 0.196 0.964
L-Cysteine HCl x H2O 0.5 g 0.49 -
Microelement solution 10.0 ml - -
Vitamin solution 10.0 ml - -
Distilled water 1000.0 ml - -
1 Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution.

Microelement solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x n H2O 0.01 g 0.01 -
ZnSO4 x 7 H2O 0.05 g 0.05 0.174
H3BO3 0.01 g 0.01 0.162
Nitrilotriacetic acid 4.50 g 4.5 23.543
CaCl2 x 2 H2O 0.01 g 0.01 0.068
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
CoCl2 x 6 H2O 0.20 g 0.2 1.54
AlK(SO4)2 x 12 H2O 0.01 g 0.01 0.021
Distilled water 1000.00 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamin 0.010 g 0.01 0.007
Ascorbic acid 0.025 g 0.025 0.142
Riboflavin 0.025 g 0.025 0.066
Citric acid 0.020 g 0.02 0.104
Pyridoxine hydrochloride 0.050 g 0.05 -
Folic acid 0.010 g 0.01 0.023
p-Aminobenzoic acid 0.010 g 0.01 0.073
Creatine 0.025 g 0.025 0.191
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 9.804 54.418
NaCl 3.922 67.104
Tryptone 3.922 55.171
Beef extract 1.961 -
K2HPO4 1.471 8.443
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Cultivation metadata from 2 strains BacDive

pH 2 - 4 1 strain
pH 4 - 6 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen anaerobe 1 strain
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