MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. J484

Download this solution as

CSV JSON PDF

Internal Solution-ID: S4241

Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.14 g 0.137 0.784
CaCl2 x 2 H2O 0.14 g 0.137 0.929
NH4Cl 0.25 g 0.244 4.56
MgSO4 x 7 H2O 3.40 g 3.317 13.458
MgCl2 x 6 H2O 2.70 g 2.634 12.957
KCl 0.33 g 0.322 4.319
NaCl 30.00 g 29.268 500.826
Iron stock solution 5.00 ml - -
Trace minerals solution 10.00 ml - -
Trace vitamins 10.00 ml - -
Na2S2O3 x 5 H2O 1.00 g 0.976 3.931
Yeast extract
(BD-Difco) 		2.00 g 1.951 -
2-Mercaptoethanol 0.40 g 0.39 4.995
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients except Na2S2O3 x 5H2O, yeast extract, Trace vitamins and 2-mercaptoethanol, and adjust pH to 6.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v). Dispense the medium into suitable culture vessels under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave 115C for 40 min. Separately autoclave Na2S2O3 x 5H2O and 2-mercaptoethanol as 50 g/L solutions and yeast extract as 100 g/L solution under a N2 atmosphere. Filter-sterilize Trace vitamins and store under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the Na2S2O3 x 5H2O, yeast extract, Trace vitamins and 2-mercaptoethanol solutions to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
J484 MODIFIED METHANOCALDOCOCCUS MEDIUM