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Solution A

Internal Solution-ID: S4260

Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 50.0 ml - -
Trace element solution SL-10 1.0 ml - -
Rumen fluid , clarified
(see Medium No. 266)
50.0 ml - -
Trypticase peptone
(BD-BBL)
1.0 g 1.092 -
Vitamin solution 5.0 ml - -
Crotonic acid 1.7 g 1.856 21.558
Resazurin 1.0 mg 0.001 0.005
Distilled water 810.0 ml - -
J499 SULFATE-FREE SYNTROPHOMONUS MEDIUM