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JCM 499 . SULFATE-FREE SYNTROPHOMONUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J499 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 916 ml - -
Solution B 70 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Mix ingredients of Solution A, adjust pH to 7.2, bring to a boil for a few minutes and cool down to room temperature under a stream of N2-CO2 (4:1, v/v) gas mixture. Distribute the solution into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize Solution B and equilibrate by gassing with the same gas mixture for at least 15 min. Autoclave Solutions C and D under a N2 atmosphere. Add appropriate amounts of Solutions B, C and D to Solution A to complete the medium.

Solution A #

916
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 50.0 ml - -
Trace element solution SL-10 1.0 ml - -
Rumen fluid , clarified
(see Medium No. 266) 		50.0 ml - -
Trypticase peptone
(BD-BBL) 		1.0 g 1.092 -
Vitamin solution 5.0 ml - -
Crotonic acid 1.7 g 1.856 21.558
Resazurin 1.0 mg 0.001 0.005
Distilled water 810.0 ml - -

Solution B #

70
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 3.5 g 50 595.188
Distilled water 70.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.3 g 30 170.808
Distilled water 10.0 ml - -

Solution D #

10
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium 335).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 10.0 g 10 73.484
MgCl2 x 6 H2O 6.6 g 6.6 32.464
NaCl 8.0 g 8 136.893
NH4Cl 8.0 g 8 149.561
CaCl2 x 2 H2O 1.0 g 1 6.802
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.25 mg 2.5e-4 0.001
Nicotinic acid 2.50 mg 0.003 0.02
Thiamine HCl 1.25 mg 0.001 0.004
p-Aminobenzoic acid 1.25 mg 0.001 0.009
Pantothenic acid 0.62 mg 6.2e-4 0.003
Pyridoxine hydrochloride 6.20 mg 0.006 0.03
Distilled water 1000.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Metadata

Last modified: 03.04.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.5 41.663
Crotonic acid 1.7 19.747
Trypticase peptone 1 -
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain