Main sol. J514
Internal Solution-ID: S4300
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 0.15 | g | 0.15 | 0.833 | |
| Yeast extract | 1.00 | g | 1 | - | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| CaCO3 | 0.10 | g | 0.1 | 0.999 | |
| Ca(NO3)2 | 0.10 | g | 0.1 | 0.609 | |
| KCl | 0.05 | g | 0.05 | 0.671 | |
| K2HPO4 | 0.05 | g | 0.05 | 0.287 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| Na2S x 9 H2O | 0.20 | g | 0.2 | 0.833 | |
| Vitamin solution | 10.00 | ml | - | - | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 990.00 | ml | - | - | |
| 1 Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution. | |||||
Media with this solution
| J514 | GLUCOSE SULFIDE MEDIUM |