1
Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (80:20) gas mixture. Distribute Solution A into the culture vessels (e.g., 9 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. To complete the medium, add 10 mM (final) sodium pyruvate solution and 0.1 volume of 3.5% NaHCO3 solution [filter-sterilized and stored under a N2-CO2 (80:20) gas mixture] and reduce the medium with 0.3 g/L (final) L-cysteine x HCl x H2O and 0.3 g/L (final) Na2S x 9H2O (autoclave and store 3% stock solutions under a N2 atmosphere).
We are using cookies to improve the user experience. We save the following information: user preferences for language, accessibility, help features and medium favourites. Unfortunately, you cannot use this site if you don't agree.