J539
539 .
MODIFIED SYNTHROPHOMONAS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
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Equipment needed: Autoclave
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution | 50.0 | ml | - | - | |
| FeCl2 solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Vitamin solution | 5.0 | ml | - | - | |
| Glucose | 20.0 | mM | 3.603 | 20 | |
| Yeast extract | 1.0 | g | 1.109 | - | |
| Peptone | 0.5 | g | 0.554 | - | |
| Na2SO4 | 2.8 | g | 3.104 | 21.855 | |
| Resazurin | 1.0 | mg | 0.001 | 0.005 | |
| Distilled water | 845.0 | ml | - | - | |
| 1 Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (80:20) gas mixture. Distribute Solution A into the culture vessels (e.g., 9 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. To complete the medium, add 10 mM (final) sodium pyruvate solution and 0.1 volume of 3.5% NaHCO3 solution [filter-sterilized and stored under a N2-CO2 (80:20) gas mixture] and reduce the medium with 0.3 g/L (final) L-cysteine x HCl x H2O and 0.3 g/L (final) Na2S x 9H2O (autoclave and store 3% stock solutions under a N2 atmosphere). | |||||
Solution occurs in 2
other media (first defined in Medium J519).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 10.0 | g | 10 | 73.483 |
| MgCl2 x 6 H2O | 6.6 | g | 6.6 | 32.464 |
| NaCl | 8.0 | g | 8 | 136.893 |
| NH4Cl | 8.0 | g | 8 | 149.561 |
| CaCl2 x 2 H2O | 1.0 | g | 1 | - |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 87
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| Distilled water | 990.0 | ml | - | - |
Solution occurs in 89
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnCl2 | 70 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
| H3BO3 | 6 | mg | 0.006 | 0.097 |
| CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium J519).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 0.25 | mg | 2.5e-4 | 0.001 |
| Nicotinic acid | 2.50 | mg | 0.003 | 0.02 |
| Thiamine HCl | 1.25 | mg | 0.001 | - |
| p-Aminobenzoic acid | 1.25 | mg | 0.001 | 0.009 |
| DL-Calcium pantothenate | 0.62 | mg | 6.2e-4 | 0.001 |
| Pyridoxine hydrochloride | 6.20 | mg | 0.006 | - |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |