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Main sol. J558

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Internal Solution-ID: S4374

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 1.60 g 1.581 11.137
KH2PO4 1.00 g 0.988 7.261
NH4Cl 0.50 g 0.494 9.237
K2SO4 0.06 g 0.059 0.34
KNO3 0.51 g 0.504 4.985
Trace x 10 solution 0.10 ml - -
Se/W solution 1.00 ml - -
Mg/Ca solution 5.00 ml - -
Na benzoate solution 72.0 g/L 2.00 ml - -
7 Vitamins solution 0.50 ml - -
FeSO4 solution (5 g/L) with EDTA 3.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2 - 7.4.
2 Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO4 solution. Aseptically add all solutions to the medium.
J558 MAGNETOSPIRILLUM MEDIUM (A)